Integration of stress-related and reactive oxygen species-mediated signals by Topoisomerase VI in Arabidopsis thaliana.
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 109 (2012-10-02), Heft 40, S. 16360-16365
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Zugriff:
Environmental stress often leads to an increased production of reactive oxygen species that are involved in plastid-to-nucleus retrograde signaling. Soon after the release of singlet oxygen (102) in chloroplasts of the flu mutant of Arabidopsis, reprogramming of nuclear gene expression reveals a rapid transfer of signals from the plastid to the nucleus. We have identified extraplastidic signaling constituents involved in 102-initiated plastid-to-nucleus signaling and nuclear gene activation after mutagenizing a flu line expressing the luciferase reporter gene under the control of the promoter of a 102-responsive AAA-ATPase gene [At3g28580) and isolating second-site mutations that lead to a constitutive up-regulation of the reporter gene or abrogate its 102-dependent up-regulation. One of these mutants, caa39, turned out to be a weak mutant allele of the Topoisomerase VI (Topo VI) A-subunit gene with a single amino acid substitution. Transcript profile analysis of flu and flu caa39 mutants revealed that Topo VI is necessary for the full activation of AAA-ATPase and a set of 102-responsive transcripts in response to 102. Topo VI binds to the promoter of the AAA-ATPase and other 102-responsive genes, and hence could directly regulate their expression. Under photoinhibitory stress conditions, which enhance the production of 102 and H202, Topo VI regulates 102-responsive and H202-responsive genes in a distinct manner. These results suggest that Topo VI acts as an integrator of multiple signals generated by reactive oxygen species formed in plants under adverse environmental conditions. [ABSTRACT FROM AUTHOR]
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Integration of stress-related and reactive oxygen species-mediated signals by Topoisomerase VI in Arabidopsis thaliana.
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Autor/in / Beteiligte Person: | Simková, Klára ; Moreau, Fanny ; Pawlak, Piotr ; Vriet, Cécile ; Baruah, Aiswarya ; Alexandre, Cristina ; Hennig, Lars ; Apel, Klaus ; Lalo, Christophe |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 109 (2012-10-02), Heft 40, S. 16360-16365 |
Veröffentlichung: | 2012 |
Medientyp: | academicJournal |
ISSN: | 0027-8424 (print) |
DOI: | 10.1073/pnas.1202041109 |
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