An in vitro system for assessing the sensitivity of Plasmodium vivax to chloroquine
In: Acta Tropica, Jg. 83 (2002-07-01), Heft 1, S. 49-61
Online
academicJournal
Zugriff:
Following earlier observations on short-term culture of Plasmodium vivax, an in vitro test system has been developed for assessing the parasite''s sensitivity to chloroquine. Fresh isolates with predominantly young trophozoites are diluted 1:19 with a (v/v=1/1) mixture of RPMI 1640 and Waymouth medium. The blood-medium mixture (BMM) is inoculated into the predosed microtitre plates before incubation in a candle jar. Incubation for 30 or 42 h yielded the best results. Incubation for 18 or 24 h was generally insufficient for an adequate development of the parasites. The reading of the test is based on stage-specific differential counts in the Giemsa-stained pre-incubation and post-incubation thick films, the evaluation on log-probit analysis of drug-related inhibition of parasite development. The test system has been evaluated on 200 fresh P. vivax isolates in an area with satisfactory clinical-parasitological response to chloroquine. At 30 or 42 h incubation 121 isolates (61.5%) showed adequate control growth and yielded valid sensitivity tests. Complete inhibition of parasite development occurred within the concentration range of 40–1280 nM. The mean EC50 for 30 h of incubation was 50.3 nM, as compared to 49.7 nM with 42 h of incubation. The geometric mean cut-off concentration of parasite development was 488 nM with 30 h of incubation as against 470 nM with 42 h of incubation. [Copyright &y& Elsevier]
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An in vitro system for assessing the sensitivity of Plasmodium vivax to chloroquine
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Autor/in / Beteiligte Person: | Tasanor, Oumaporn ; Noedl, Harald ; Na-Bangchang, Kesara ; Congpuong, Kanungnit ; Sirichaisinthop, Jeeraphat ; Wernsdorfer, Walther H. |
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Zeitschrift: | Acta Tropica, Jg. 83 (2002-07-01), Heft 1, S. 49-61 |
Veröffentlichung: | 2002 |
Medientyp: | academicJournal |
ISSN: | 0001-706X (print) |
DOI: | 10.1016/S0001-706X(02)00056-6 |
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