Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae
In: Bioresource Technology, Jg. 103 (2012), Heft 1, S. 351-359
Online
academicJournal
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Abstract: In this study, an aldehyde dehydrogenase (ALDH) was over-expressed in Klebsiella pneumoniae for simultaneous production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO). Various genes encoding ALDH were cloned and expressed in K. pneumoniae, and expression of Escherichia coli aldH resulted in the highest 3-HP titer in anaerobic cultures in shake flasks. Anaerobic fed-batch culture of this recombinant strain was further performed in a 5-L reactor. The 3-HP concentration and yield reached 24.4g/L and 0.18mol/mol glycerol, respectively, and at the same time 1,3-PDO achieved 49.3g/L with a yield of 0.43mol/mol in 24h. The overall yield of 3-HP plus 1,3-PDO was 0.61mol/mol. Over-expression of the E. coli AldH also reduced the yields of by-products except for lactate. This study demonstrated the possibility of simultaneous production of 3-HP and 1,3-PDO by K. pneumoniae under anaerobic conditions without supply of vitamin B12. [Copyright &y& Elsevier]
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Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae
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Autor/in / Beteiligte Person: | Huang, Yanna ; Li, Zhimin ; Shimizu, Kazuyuki ; Ye, Qin |
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Zeitschrift: | Bioresource Technology, Jg. 103 (2012), Heft 1, S. 351-359 |
Veröffentlichung: | 2012 |
Medientyp: | academicJournal |
ISSN: | 0960-8524 (print) |
DOI: | 10.1016/j.biortech.2011.10.022 |
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