Trimethyiguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs.
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 107 (2010-08-17), Heft 33, S. 14787-14792
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Zugriff:
5'-mRNA capping is an early modification that affects pre-mRNA synthesis/splicing, RNA cytoplasmictransport, and mRNA translation and turnover. In eukaryotes. a 7-methylguanosine (m7G) cap is added to newlytranscribed RNA polymerase II (RNAP II)transcripts. A subset of RNAP Il-transcribed cellular RNAs. including small nuclear RNA (snRNA), small nucleolar RNA (snoRNA), and telomerase RNA. is further hypermethylated at the exocyclic N2 of the guanosine to create a trimethylguanosine (TMG)-capped RNA. Some of these TMG-capped RNA5 are transported within the nucleus and from the nucleus to the cytoplasm by the CRM-1 (required for chromo- some region maintenance) protein. CRM-1 is also used to export Rev/RRE-dependent unspliced/ partially spliced HIV-1 RNA5. Here we report that like snRNAs and snoRNAs, some Rev/RRE-dependent HIV-1 RNAs are TMG-capped. The methyltransferase responsible for TMG modification of HIV-1 RNAs is the human PIMT (peroxisome pro- liferator-activated receptor-interacting protein with methyltransfer- ase) protein. 1MG capping of unspliced/partially spliced HIV-1 RNA5 represents a new regulatory mechanism for selective expression. [ABSTRACT FROM AUTHOR]
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Trimethyiguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs.
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Autor/in / Beteiligte Person: | Yedavalli, Venkat S. R. K. ; Jeang, Kuan-Teh |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 107 (2010-08-17), Heft 33, S. 14787-14792 |
Veröffentlichung: | 2010 |
Medientyp: | academicJournal |
ISSN: | 0027-8424 (print) |
DOI: | 10.1073/pnas.1009490107 |
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