Effects of genetic manipulation of alcohol dehydrogenase levels on the response to stress and the synthesis of secondary metabolites in grapevine leaves.
In: Journal of Experimental Botany, Jg. 57 (2006), Heft 1, S. 91-99
Online
academicJournal
Zugriff:
The functional role of Adh in regulating susceptibility to abiotic stress and the synthesis of secondary metabolites was investigated in transgenic grapevine plants over- and underexpressing alcohol dehydrogenase (Adh). Plants were transformed with gene constructs containing a sense or antisense orientated grapevine VvAdh2 cDNA under the constitutive cauliflower mosaic virus 35S promoter. Plants transformed with either antisense orientation or the Adh-less construct displayed a low but detectable constitutive ADH activity, whereas plants transformed with the sense-expressed transgene showed a significantly higher (100-fold) ADH activity than the control. Compared with the control, the sense transgene induced an overexpression of VvAdh2 transcripts, whereas a reduced VvAdh2 expression was detected in antisense transformants. Grapevine plants overexpressing Adh displayed a lower sucrose content, a higher degree of polymerization of proanthocyanidins, and a generally increased content of volatile compounds, mainly in carotenoid- and shikimate-derived volatiles. In general, no significant differences between sense/antisense transformants were observed with regard to carotenoid and chlorophyll contents, suggesting a strong metabolic regulation of the synthesis of these compounds. [ABSTRACT FROM PUBLISHER]
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Effects of genetic manipulation of alcohol dehydrogenase levels on the response to stress and the synthesis of secondary metabolites in grapevine leaves.
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Autor/in / Beteiligte Person: | Tesniere, Catherine ; Torregrosa, Laurent ; Pradal, Martine ; Souquet, Jean-Marc ; Gilles, Christine ; Dos Santos, Karine ; Chatelet, Philippe ; Gunata, Ziya |
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Zeitschrift: | Journal of Experimental Botany, Jg. 57 (2006), Heft 1, S. 91-99 |
Veröffentlichung: | 2006 |
Medientyp: | academicJournal |
ISSN: | 0022-0957 (print) |
DOI: | 10.1093/jxb/erj007 |
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