Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability.
In: International Journal of Molecular Sciences, Jg. 9 (2008-12-01), Heft 12, S. 2366-2375
Online
academicJournal
Zugriff:
Deoxynivalenol (DON) is one of the most common contaminants of various foodstuffs. A biotransformation system was used in order to lessen the toxicity of DON. A strain of Aspergillus (NJA-1) was isolated from soil and cultured in an inorganic salt medium containing DON. Bt2a/Bt2b primers were used to amplify the β-tubulin gene of NJA-1. Sequence analysis the PCR product and morphology observation indicated that NJA-1 belonged to Aspergillus tubingensis (aerobic fungi). The DNA sequence information of the PCR product was deposited in GenBank (accession number DQ9025790). The DNA sequence had 99% similarity to the Aspergillus tubingensis accession number AY820009. An unknown compound in NJA-1 showed the ability to convert DON into another product. The molecular weight of the bioconversion product was 18.1 D (H2O) larger than that of DON. The analysis showed that DON could be hydrolyzed by NJA-1. The mean DON biotransformation rate was 94.4% after two weeks of cultivation. The finding presents a new method for DON biotransformation. [ABSTRACT FROM AUTHOR]
Titel: |
Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability.
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Autor/in / Beteiligte Person: | He, Chenghua ; Fan, Yanhong ; Liu, Guofang ; Zhang, Haibin |
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Zeitschrift: | International Journal of Molecular Sciences, Jg. 9 (2008-12-01), Heft 12, S. 2366-2375 |
Veröffentlichung: | 2008 |
Medientyp: | academicJournal |
ISSN: | 1661-6596 (print) |
DOI: | 10.3390/ijms9122366 |
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