Stimulatory and suppressive signal transduction regulates vasoactive intestinal peptide receptor-1 (VPAC-1) in primary mouse CD4 T cells
In: Brain, Behavior & Immunity, Jg. 22 (2008-10-01), Heft 7, S. 1024-1031
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Abstract: Vasoactive intestinal peptide receptor-1 (VPAC-1) is an anti-proliferative, G-protein coupled receptor that is highly expressed on naïve T cells, and has been reported to be downregulated upon T cell activation. The T cell signaling molecules involved in mediating low VPAC-1 levels have not been identified. Therefore, to gain a greater understanding into this regulation, this study investigated the signaling pathways that regulate (VPAC-1) in murine, primary CD4 T cells. To this end, murine, splenic CD4 T cells were pretreated separately with 10 different pharmacological inhibitors and incubated +/− anti-CD3 for 24h. Total RNA was isolated, and VPAC-1 mRNA levels were measured by qPCR. Our results support that JNK kinases, downstream from the protein kinase, Zap70, are involved in suppressive regulation of VPAC-1 steady-state mRNA levels after anti-CD3 treatment. In contrast, inhibitors against PKC, ERK, p38, Zap70 and Rac1 supported a stimulatory influence in VPAC-1 regulation in the absence of T cell signaling. By studying the signaling pathways that regulate VPAC-1 in T cells, we can gain greater insight into the role of this anti-inflammatory receptor in autoimmunity and infectious diseases. [Copyright &y& Elsevier]
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Stimulatory and suppressive signal transduction regulates vasoactive intestinal peptide receptor-1 (VPAC-1) in primary mouse CD4 T cells
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Autor/in / Beteiligte Person: | Vomhof-DeKrey, Emilie E. ; Dorsam, Glenn Paul |
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Zeitschrift: | Brain, Behavior & Immunity, Jg. 22 (2008-10-01), Heft 7, S. 1024-1031 |
Veröffentlichung: | 2008 |
Medientyp: | academicJournal |
ISSN: | 0889-1591 (print) |
DOI: | 10.1016/j.bbi.2008.04.006 |
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