Multiphoton FLIM Analyses of Native and UVA-Modified Synthetic Melanins.
In: International Journal of Molecular Sciences, Jg. 24 (2023-03-01), Heft 5, S. 4517-4550
Online
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Zugriff:
To better understand the impact of solar light exposure on human skin, the chemical characterization of native melanins and their structural photo-modifications is of central interest. As the methods used today are invasive, we investigated the possibility of using multiphoton fluorescence lifetime (FLIM) imaging, along with phasor and bi-exponential fitting analyses, as a non-invasive alternative method for the chemical analysis of native and UVA-exposed melanins. We demonstrated that multiphoton FLIM allows the discrimination between native DHI, DHICA, Dopa eumelanins, pheomelanin, and mixed eu-/pheo-melanin polymers. We exposed melanin samples to high UVA doses to maximize their structural modifications. The UVA-induced oxidative, photo-degradation, and crosslinking changes were evidenced via an increase in fluorescence lifetimes along with a decrease in their relative contributions. Moreover, we introduced a new phasor parameter of a relative fraction of a UVA-modified species and provided evidence for its sensitivity in assessing the UVA effects. Globally, the fluorescence lifetime properties were modulated in a melanin-dependent and UVA dose-dependent manner, with the strongest modifications being observed for DHICA eumelanin and the weakest for pheomelanin. Multiphoton FLIM phasor and bi-exponential analyses hold promising perspectives for in vivo human skin mixed melanins characterization under UVA or other sunlight exposure conditions. [ABSTRACT FROM AUTHOR]
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Multiphoton FLIM Analyses of Native and UVA-Modified Synthetic Melanins.
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Autor/in / Beteiligte Person: | Pena, Ana-Maria ; Ito, Shosuke ; Bornschlögl, Thomas ; Brizion, Sébastien ; Wakamatsu, Kazumasa ; Del Bino, Sandra |
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Zeitschrift: | International Journal of Molecular Sciences, Jg. 24 (2023-03-01), Heft 5, S. 4517-4550 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 1661-6596 (print) |
DOI: | 10.3390/ijms24054517 |
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