Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method.
In: Microbial Cell Factories, Jg. 18 (2019-08-06), Heft 1, S. N.PAG
Online
academicJournal
Zugriff:
Background: Acetoin (AC) and 2,3-butanediol (2,3-BD) as highly promising bio-based platform chemicals have received more attentions due to their wide range of applications. However, the non-efficient substrate conversion and mutually transition between AC and 2,3-BD in their natural producing strains not only led to a low selectivity but also increase the difficulty of downstream purification. Therefore, synthetic engineering of more suitable strains should be a reliable strategy to selectively produce AC and 2,3-BD, respectively. Results: In this study, the respective AC (alsS and alsD) and 2,3-BD biosynthesis pathway genes (alsS, alsD, and bdhA) derived from Bacillus subtilis 168 were successfully expressed in non-natural AC and 2,3-BD producing Corynebacterium crenatum, and generated recombinant strains, C. crenatum SD and C. crenatum SDA, were proved to produce 9.86 g L−1 of AC and 17.08 g L−1 of 2,3-BD, respectively. To further increase AC and 2,3-BD selectivity, the AC reducing gene (butA) and lactic acid dehydrogenase gene (ldh) in C. crenatum were then deleted. Finally, C. crenatumΔbutAΔldh SD produced 76.93 g L−1 AC in one-step biocatalysis with the yield of 0.67 mol mol−1. Meanwhile, after eliminating the lactic acid production and enhancing 2,3-butanediol dehydrogenase activity, C. crenatumΔldh SDA synthesized 88.83 g L−1 of 2,3-BD with the yield of 0.80 mol mol−1. Conclusions: The synthetically engineered C. crenatumΔbutAΔldh SD and C. crenatumΔldh SDA in this study were proved as an efficient microbial cell factory for selective AC and 2,3-BD production. Based on the insights from this study, further synthetic engineering of C. crenatum for AC and 2,3-BD production is suggested. [ABSTRACT FROM AUTHOR]
Titel: |
Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method.
|
---|---|
Autor/in / Beteiligte Person: | Zhang, Xian ; Han, Rumeng ; Bao, Teng ; Zhao, Xiaojing ; Li, Xiangfei ; Zhu, Manchi ; Yang, Taowei ; Xu, Meijuan ; Shao, Minglong ; Zhao, Youxi ; Rao, Zhiming |
Link: | |
Zeitschrift: | Microbial Cell Factories, Jg. 18 (2019-08-06), Heft 1, S. N.PAG |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 1475-2859 (print) |
DOI: | 10.1186/s12934-019-1183-0 |
Schlagwort: |
|
Sonstiges: |
|