Early protection against pathogenic virus infection at a mucosal challenge site after vaccination with attenuated simian immunodeficiency virus.
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 101 (2004-03-02), Heft 9, S. 3017-3022
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Zugriff:
Atraumatic application of attenuated SlVmac239Δnef vaccine to the tonsils of rhesus macaques provided protection against challenge 26 weeks later with infectious SIVmac25l applied through this route. Early events at the mucosal portal of entry of challenge virus were followed. Wild-type virus was detected in nonvacci- nated controls by day 4, and then simian immunodeficiency virus (SIV) replicated vigorously at days 7 and 14. In contrast, a challenge of 10 of 10 vaccinees with SIV did not significantly raise RNA levels in the plasma or increase infected cells in lymphoid tissues, as assessed by single-cell labeling for viral RNA and nef protein. Vaccine virus was found in the tonsils of all vaccinees, but challenge virus was only detected at this portal of entry in 4 of 10 monkeys. In the tonsil, the challenge virus did not induce an expansion of perforin+ killer cells. However, there was a significant increase in γ ö T cells and mature dendritic cells relative to unvaccinated controls. Therefore, during tonsillar SlVΔnef vaccination, infection is blocked early at the entry portal, which we propose is due in part to innate functions of γ ö T and dendritic cells. [ABSTRACT FROM AUTHOR]
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Early protection against pathogenic virus infection at a mucosal challenge site after vaccination with attenuated simian immunodeficiency virus.
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Autor/in / Beteiligte Person: | Tenner-Racz, Kiara ; Hennig, Christiane Stahl ; Überla, Klaus ; Stoiber, Heribert ; Ignatius, Raif ; Heeney, Jonathan ; Steinman, Ralph M. ; Racz, Paul |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 101 (2004-03-02), Heft 9, S. 3017-3022 |
Veröffentlichung: | 2004 |
Medientyp: | academicJournal |
ISSN: | 0027-8424 (print) |
DOI: | 10.1073/pnas.0308677101 |
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