Vasoactive intestinal peptide increases vascular endothelial growth factor expression and neuroendocrine differentiation in human prostate cancer LNCaP cells
In: Regulatory Peptides, Jg. 119 (2004-06-15), Heft 1/2, S. 69-75
Online
academicJournal
Zugriff:
Vasoactive intestinal peptide (VIP) upregulates the expression of vascular endothelial cell growth factor (VEGF189, VEGF165 and VEGF121) mRNAs in human prostate cancer LNCaP cells, as shown by reverse transcriptase-polymerase chain reaction (RT-PCR). Real-time RT-PCR indicated that the effect was maximal by 1–2 h and must be accounted for increased transcription since VIP decreased VEGF165 mRNA stability. VIP stimulated VEGF165 protein synthesis as measured by ELISA. VIP regulation of VEGF expression was mediated by VPAC1 receptor and was cAMP/protein kinase A (PKA) dependent. Phosphoinositide 3-kinase (PI3-K) and mitogen-activated protein kinase MEK1/2 systems may also be involved as shown with specific kinase inhibitors. These actions together with the observation of VIP-induced neuroendocrine differentiation in LNCaP cells suggest a proangiogenic potential of VIP in prostate cancer. [Copyright &y& Elsevier]
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Vasoactive intestinal peptide increases vascular endothelial growth factor expression and neuroendocrine differentiation in human prostate cancer LNCaP cells
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Autor/in / Beteiligte Person: | Collado, Beatriz ; Gutiérrez-Cañas, Irene ; Rodríguez-Henche, Nieves ; Prieto, Juan C. ; Carmena, María J. |
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Zeitschrift: | Regulatory Peptides, Jg. 119 (2004-06-15), Heft 1/2, S. 69-75 |
Veröffentlichung: | 2004 |
Medientyp: | academicJournal |
ISSN: | 0167-0115 (print) |
DOI: | 10.1016/j.regpep.2004.01.013 |
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