A New Method Based on the Use of Dicthyl Pyrocarbonate as a Nuclease Inhibitor for the Extraction of Undergraded Nucleic Acid from Plant Tissues.
In: European Journal of Biochemistry, Jg. 5 (1968-09-01), Heft 4, S. 520-527
academicJournal
Zugriff:
A new method, based on the use of diethyl pyrocarbonate as a, nuclease inhibitor, has been worked out for the extraction of undegraded nucleic acids from etiolated and green plant tissues. Experimental evidence is given to show that the nucleic acids isolated by this method exhibit a high degree of purity as evidenced by their spectra and that they are essentially undegraded as indicated by sedimentation patterns in sucrose density gradients. [ABSTRACT FROM AUTHOR]
Titel: |
A New Method Based on the Use of Dicthyl Pyrocarbonate as a Nuclease Inhibitor for the Extraction of Undergraded Nucleic Acid from Plant Tissues.
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Autor/in / Beteiligte Person: | Solymosy, F. ; Fedorcsäk, I. ; Gulyäs, A. ; Farkas, G. L. ; Ehrenberg, L. |
Zeitschrift: | European Journal of Biochemistry, Jg. 5 (1968-09-01), Heft 4, S. 520-527 |
Veröffentlichung: | 1968 |
Medientyp: | academicJournal |
ISSN: | 0014-2956 (print) |
DOI: | 10.1111/j.1432-1033.1968.tb00401.x |
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