Enantioselective cascade biocatalysis for deracemization of 2-hydroxy acids using a three-enzyme system.
In: Microbial Cell Factories, Jg. 15 (2016-09-22), S. 1-9
Online
academicJournal
Zugriff:
Background: Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. Results: The method was realized by a single recombinant Escherichia coli strain coexpressing three enzymes: (S)- 2-hydroxy acid dehydrogenase, (R)-2-keto acid reductase and glucose dehydrogenase. One enantiomer [(S)-2-hydroxy acid] is firstly oxidized to the keto acid with (S)-2-hydroxy acid dehydrogenase, while the other enantiomer [(R)-2-hydroxy acid] remains unchanged. Then, the keto acid obtained reduced to the opposite enantiomer with (R)-2-keto acid reductase plus cofactor regeneration enzyme glucose dehydrogenase subsequently. The recombinant E. coli strain coexpressing the three enzymes was proven to be a promising biocatalyst for the cascade bioconversion of a structurally diverse range of racemic 2-hydroxy acids, giving the corresponding (R)-2-hydroxy acids in up to 98.5 % conversion and >99 % enantiomeric excess. Conclusions: In summary, a cascade biocatalysis was successfully developed to prepare valuable (R)-2-hydroxy acids with an efficient three-enzyme system. The developed elegant cascade biocatalysis possesses high atom efficiency and represents a promising strategy for production of highly valued (R)-2-hydroxy acids. [ABSTRACT FROM AUTHOR]
Titel: |
Enantioselective cascade biocatalysis for deracemization of 2-hydroxy acids using a three-enzyme system.
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Autor/in / Beteiligte Person: | Xue, Ya-Ping ; Zeng, Hao ; Jin, Xiao-Lu ; Liu, Zhi-Qiang ; Zheng, Yu-Guo |
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Zeitschrift: | Microbial Cell Factories, Jg. 15 (2016-09-22), S. 1-9 |
Veröffentlichung: | 2016 |
Medientyp: | academicJournal |
ISSN: | 1475-2859 (print) |
DOI: | 10.1186/s12934-016-0560-1 |
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