Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response.
In: PLoS Pathogens, Jg. 12 (2016-07-25), Heft 7, S. 1-35
Online
academicJournal
Zugriff:
Spliceosomal SNRNP200 is a Ski2-like RNA helicase that is associated with retinitis pigmentosa 33 (RP33). Here we found that SNRNP200 promotes viral RNA sensing and IRF3 activation through the ability of its amino-terminal Sec63 domain (Sec63-1) to bind RNA and to interact with TBK1. We show that SNRNP200 relocalizes into TBK1-containing cytoplasmic structures upon infection, in contrast to the RP33-associated S1087L mutant, which is also unable to rescue antiviral response of SNRNP200 knockdown cells. This functional rescue correlates with the Sec63-1-mediated binding of viral RNA. The hindered IFN-β production of knockdown cells was further confirmed in peripheral blood cells of RP33 patients bearing missense mutation in SNRNP200 upon infection with Sendai virus (SeV). This work identifies a novel immunoregulatory role of the spliceosomal SNRNP200 helicase as an RNA sensor and TBK1 adaptor for the activation of IRF3-mediated antiviral innate response. [ABSTRACT FROM AUTHOR]
Titel: |
Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response.
|
---|---|
Autor/in / Beteiligte Person: | Tremblay, Nicolas ; Baril, Martin ; Chatel-Chaix, Laurent ; Es-Saad, Salwa ; Park, Alex Young ; Koenekoop, Robert K. ; Lamarre, Daniel |
Link: | |
Zeitschrift: | PLoS Pathogens, Jg. 12 (2016-07-25), Heft 7, S. 1-35 |
Veröffentlichung: | 2016 |
Medientyp: | academicJournal |
ISSN: | 1553-7366 (print) |
DOI: | 10.1371/journal.ppat.1005772 |
Schlagwort: |
|
Sonstiges: |
|