Thrombomodulin-mediated Cell Adhesion.
In: Journal of Biological Chemistry, Jg. 278 (2003-11-21), Heft 47, S. 46750-46759
academicJournal
Zugriff:
Thrombomodulin (TM) is an integral membrane glycoprotein that is a potent anticoagulant factor. TM may also possess functions distinct from its anticoagulant activity. Here the influence of TM on cell adhesion was studied in TM-negative melanoma A2058 cells transfected with green fluorescent protein-tagged TM (TMG) or lectin domain-deleted TM (TMG(ΔL)). Confocal microscopy demonstrated that both TMG and TMG(ΔL) were distributed in the plasma membrane. TMG-expressed cells grew as closely clustered colonies, with TM localized prominently in the intercellular boundaries. TMG(ΔL)-expressed cells grew singly. Overexpression of TMG, but not TMG(ΔL), decreased monolayer permeability in vitro and tumor growth in vivo. The cell-to-cell adhesion in TMG-expressed cells was Ca[sup 2+]-dependent and was inhibited by monoclonal antibody against the lectin-like domain of TM. The effects of TM-mediated cell adhesion were abolished by the addition of mannose, chondroitin sulfate A, or chondroitin sulfate C. In addition, anti-lectin-like domain antibody disrupted the close clustering of the endogenous TM-expressed keratinocyte HaCaT cell line derived from normal human epidermis. Double-labeling immunofluorescence staining revealed similar distributions of TM and actin filament in the cortex region of the TMG-expressed cells. Thus, TM can function as a Ca[sup 2+]-dependent cell-to-cell adhesion molecule. Binding of specific carbohydrates to the lectin-like domain is essential for this specific function. [ABSTRACT FROM AUTHOR]
Titel: |
Thrombomodulin-mediated Cell Adhesion.
|
---|---|
Autor/in / Beteiligte Person: | Huey-Chun Huang, Rafael ; Guey-Yueh Shi, Rafael ; Shinn-Jong Jiang, Rafael ; Chung-Sheng Shi, Rafael ; Chun-Mei Wu, Rafael ; Yang, Hsi-Yuan ; Hua-Lin Wu, Rafael |
Zeitschrift: | Journal of Biological Chemistry, Jg. 278 (2003-11-21), Heft 47, S. 46750-46759 |
Veröffentlichung: | 2003 |
Medientyp: | academicJournal |
ISSN: | 0021-9258 (print) |
DOI: | 10.1074/jbc.M305216200 |
Schlagwort: |
|
Sonstiges: |
|