Multicentre evaluation of a real-time PCR assay to detect genes encoding clinically relevant carbapenemases in cultured bacteria.
In: International Journal of Antimicrobial Agents, Jg. 47 (2016-02-01), Heft 2, S. 151-154
Online
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Zugriff:
The performance and portability of a multiplex real-time PCR assay to detect KPC, NDM, OXA-48-like and VIM carbapenemase gene families from bacterial isolates was assessed using Rotor-Gene Q and ABI 7500 instruments. Gram-negative bacterial isolates ( n = 502) were comprised of 100 isolates each with KPC, NDM, VIM or OXA-48-like carbapenemases (including 17 with OXA-181) and 2 isolates with NDM + OXA-48-like enzymes (including 1 with OXA-181) as well as 100 assay-negative isolates comprised of 24 IMP-producers, 24 carbapenem-resistant isolates with no known carbapenemase gene and 52 extended-spectrum β-lactamase-producing carbapenem-susceptible isolates. A multicentre evaluation was carried out in five laboratories using a subset of 100 isolates comprised of 22 isolates each with KPC, NDM, OXA-48-like or VIM alleles and 12 isolates that were negative for the assay targets. Initial validation of the assay on both the Rotor-Gene Q and ABI 7500 instruments demonstrated 100% sensitivity amongst the 402 isolates that were positive for KPC, NDM, OXA-48-like (including OXA-181) and VIM carbapenemase genes, whilst the 100 assay-negative samples were correctly identified indicating 100% specificity. During the multicentre evaluation the same 100% level of sensitivity and specificity was observed in each of the five centres that participated. Neither invalid nor false-positive results were observed. In conclusion, the assay offers a portable and reliable option for the detection of bacteria carrying clinically significant carbapenemases encoded by KPC, NDM, VIM and OXA-48-like carbapenemase genes using either of the two most common real-time PCR instrument platforms. [ABSTRACT FROM AUTHOR]
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Multicentre evaluation of a real-time PCR assay to detect genes encoding clinically relevant carbapenemases in cultured bacteria.
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Autor/in / Beteiligte Person: | Ellington, Matthew J. ; Findlay, Jacqueline ; Hopkins, Katie L. ; Meunier, Danièle ; Alvarez-Buylla, Adela ; Horner, Carolyne ; McEwan, Ashley ; Guiver, Malcolm ; McCrae, Li-Xu ; Woodford, Neil ; Hawkey, Peter |
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Zeitschrift: | International Journal of Antimicrobial Agents, Jg. 47 (2016-02-01), Heft 2, S. 151-154 |
Veröffentlichung: | 2016 |
Medientyp: | academicJournal |
ISSN: | 0924-8579 (print) |
DOI: | 10.1016/j.ijantimicag.2015.11.013 |
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