VPLIV DODATKA HMELJA NA OKSIDACIJO KRMNIH MEŠANIC IN OKSIDATIVNI STRES PIŠČANCEV PITANCEV.

The influence of the addition of hop cones on oxidative stability of feed mixtures during their storage and on oxidative status of broilers was investigated. 84 chickens Ross 308 were divided in 3 experimental groups in the nutritional experiment. With feed the chickens consumed 7.5% linseed oil, which is a rich source of polyunsaturated fatty acids (PUFA), thereby causing an increase in oxidative stress. No hops (control group, KONT), 0.9 (HMELJ_0,9) or 3.6 (HMELJ_3,6) hops g/kg feed was added in feed mixtures. Stability testing of - and -acids in hops and feed mixtures during aging has shown that storage temperature and hop variety are very important factors. We monitored the oxidation of unsaturated fatty acids in feed mixtures and effect of hop addition on the oxidation of lipids in the chickens blood plasma, which was evaluated by monitoring the concentration of malondialdehyde (MDA), a secondary product of the oxidation of polyunsaturated fatty acids. In feed mixtures finisher higher concentration of MDA was measured in comparison to feed mixtures starter. The highest concentration of MDA was determined in the feed mixture HMELJ_3,6, which induced oxidation of lipids and increased the concentration of MDA in the blood plasma of chickens. Depending on the content of MDA in feed mixtures and in the chickens' blood plasma, we found that in the PUFA enriched feed, hop acts as prooxidant. [ABSTRACT FROM AUTHOR]

Raziskali smo vpliv dodatka hmeljevih storžkov na oksidacijsko stabilnost krmnih mešanic med njihovim skladiščenjem in na oksidativni status pitovnih piščancev. V prehranskem poskusu smo 84 piščancev pitancev ross 308 razdelili v 3 poskusne skupine. S krmo so piščanci zaužili 7,5% lanenega olja, ki je bogat vir večkrat nenasičenih maščobnih kislin (VNMK) in s tem povzročili povečan oksidacijski stres. V krmne mešanice smo dodali nič (kontrolna skupina, KONT) oz. 0,9 (HMELJ_0,9) ali 3,6 (HMELJ_3,6) g hmelja/kg krmne mešanice. Poskus obstojnosti - in -kislin v hmelju in krmnih mešanicah med staranjem je pokazal, da je zelo pomembna temperatura skladiščenja in sorta hmelja. Spremljali smo oksidacijo nenasičenih maščobnih kislin v krmnih mešanicah ter vpliv dodatka hmelja na oksidacijo lipidov v krvni plazmi piščancev, ki smo jo ovrednotili s spremljanjem koncentracije malondialdehida (MDA), sekundarnega produkta oksidacije večkrat nenasičenih maščobnih kislin. V krmnih mešanicah finišer smo izmerili višjo koncentracijo MDA kot v krmnih mešanicah štarter. Najvišjo koncentracijo MDA smo določili v skupini HMELJ_3,6, kar je posledično vplivalo tudi na povečano oksidacijo lipidov in koncentracijo MDA v krvni plazmi piščancev. Glede na vsebnost MDA v krmnih mešanicah in v krvni plazmi piščancev smo ugotovili, da v krmnih mešanicah, obogatenih z VNMK, hmelj deluje prooksidativno. [ABSTRACT FROM AUTHOR]