The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolysis of p-nitrophenyl glycosides.
In: Carbohydrate Research, Jg. 412 (2015-08-14), S. 43-49
Online
academicJournal
Zugriff:
In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters k cat , K m and product K I for the hydrolysis of a p- nitrophenyl glycoside by an exo -acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo -glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidase from Penicillium sp. and α-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated α- d -galactose, affected the reaction significantly. [ABSTRACT FROM AUTHOR]
Titel: |
The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolysis of p-nitrophenyl glycosides.
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Autor/in / Beteiligte Person: | Borisova, Anna S. ; Reddy, Sumitha K. ; Ivanen, Dina R. ; Bobrov, Kirill S. ; Eneyskaya, Elena V. ; Rychkov, Georgy N. ; Sandgren, Mats ; Stålbrand, Henrik ; Sinnott, Michael L. ; Kulminskaya, Anna A. ; Shabalin, Konstantin A. |
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Zeitschrift: | Carbohydrate Research, Jg. 412 (2015-08-14), S. 43-49 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 0008-6215 (print) |
DOI: | 10.1016/j.carres.2015.03.021 |
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